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We examined 12 long-term studies (of more than 90 days, up to 2 years in duration) and 12 multigenerational studies (from 2 to 5 generations).
The selection was started therefore at 8% O2 and this concentration was gradually decreased by 1% each 3 to 5 generations to keep the selection pressure.
Therefore, hypoxia selection was initiated at 8% O2, and the O2 concentration was gradually decreased by ∼1% every 3 to 5 generations to maintain selection pressure.
Time-lapse microscopy and imaging was performed to observe cell divisions in developing micro-colonies on Zeiss Imager (Axio Imager Z1) microscope with the magnification of 63× OEL objective and Differential Interference Contrast (DIC) technique in transmitted light till the microcolonies reached 16 to 32 cells stage, i e. 4 to 5 generations (Figure S1).
The number of generations per pedigree varies from 2 to 5 generations (Figure 1) which seem to account for the large range of number of nuclear families per pedigree and the distribution of inheritance vector bits seen in the data (Figure 2).
Human-to-human transmission up to 5 generations was described.
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We generally repeat this procedure for six to 10 generations.
Over up to ∼5 generations of full actinide recycle in SFR burners, the final core inventory tends to dominate over reprocessing losses, beyond which the radiotoxicity rapidly becomes sensitive to reprocessing losses.
Devoted Aunt Fannie to 3 generations.
Armyworms complete about 3 to 4 generations per year.
Cereal aphids complete about 10 to 20 generations per year.
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