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On the basis of the criteria described above, 77 publications were discarded (see SDC Table 2) and 14 studies, including 1 that was analyzed according to 2 subgroups, totaling 15 different series, were included (Tables 1 and 2).
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Goats of each genotype were allocated to 3 subgroups and fed 3 diets ad libitum in a 3 × 3 Latin square design.
Models for 1 to 15 subgroups were examined.
As in other plants, Populus PIPs and TIPs have two (PtPIP1 and PtPIP2) and five (PtTIP1 to PtTIP5) subgroups respectively.
Sixty rats were randomly assigned to 10 subgroups of six rats each, and the 10 subgroups were randomly divided into two major groups (WL and NL groups) composed of five subgroups.
By the criterion that gene sequences having 75%% or greater of nucleotide identity belong to the same subgroup, the TRAV genes belong to 34 subgroups and the TRDV genes to four subgroups (Additional file 2).
Using gene expression profiling, Gatza and colleagues showed that up to 12 subgroups with discriminating molecular profiles can be identified in ER-positive breast cancer [ 2].
Signature amino acids specific to B2 subgroup of ERF proteins are highlighted.
Our phylogenetic analysis reveals three clear subgroups: subgroup 1 (MOTUs 18 to 20), subgroup 2 (MOTUs 21 and 22), and subgroup 3 (MOTUs 23 to 27).
As expected, all of the TRAV genes belonged to the identified subgroups: 16 clones contain TRAV14 subgroup genes (series V14UM from spleen and series V14UT from thymus); 13 clones correspond to TRAV21 subgroup genes (series V21UM from spleen and series V21UT from thymus); 19 clones match with TRAV25 subgroup genes (series V25UM from spleen and series V25UT from thymus).
As to fly RBF1, RBF2, and worm LIN35, as all of them have similar evolutionary distance to RB1 subgroup and RBL subgroup proteins, we do not classify them into any subgroup.
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