In addition to L19, we studied F16 (specific to the extra-domain A1 of TnC; [ 10, 35]), G11 (specific to the extra-domain C of TnC; [ 36, 37]) and F8 (specific to the extra-domain A (EDA) of fibronectin; [ 38]).
We detected the previously reported mesenchymal-enriched expression of Fgf7, Tbx15, and Tnc [ 17, 29- 31].
Six of the eight genes examined, namely Kif5b, Ptger2, Grb10, Tnc and Dkkl1, were differentially expressed between the two strains.
b Trigeminal nucleus caudalis (TNC), ×200, scale bar 150 μc.
C-fos immunoreactivity is a widely used marker of neuronal activity in the TNC [33, 34].
The third FnIII domain of TNC (TNfn3) contains an RGD sequence and is known to bind integrins.
In control group a-c of the TNC, Nrf2 is present mainly in the cytoplasm (shown by arrows).
In previous studies, intracisternal capsaicin evoked c-fos expression over the rostro-caudal extent of the TNC [30, 31].
KATPchannels are expressed in migraine-related structures such as the cranial arteries, TG and TNC [18 22, 66].
Subcutaneous injection of sumatriptan had no significant effect on the IS-induced c-fos up-regulation (Fig. 1a; 6.5 ± 1.8 in the rostral TNC, 28.2 ± 5.3 in the caudal TNC, 25 ± 1.3 at C1, 18.8 ± 2.7 at C2; p ≥ 0.18 compared to IS + vehicle at all levels, ANOVA with Bonferroni correction; n = 5).
