Sentence examples for tm steps from inspiring English sources

The two possible TM steps shown in Scheme 2 are expected to show quite different behavior with respect to the electron density on the nucleophile, since one occurs at FeI and the other at FeIII.

Adding a step in the bottom panel to search for genes returned by a SignalP search, and using the Venn diagram to construct the union of these results with the TM step, yields an additional 2525 genes in the entire database (11 729 total), contributing four more genes to the final result in the top panel.

Immunohistochemical staining was performed using the Superision TM two-step method.

A sample was only considered positive if both the sample quantification curve became exponential, and the PCR product was correctly differentiated by the Tm analysis step.

Total RNA was used to amplify HIF-1α/VEGF/β-actin using the Superscript TM One Step RT-PCR with Platinum®Taq (Invitrogen, Sydney, Australia).

RNA was extracted from serum using silica-membrane-based nucleic acid purification kit (QIAmp viral RNA, Qiagen, Germany), RNA was reverse transcribed (Superscript TM One step RT PCR Platinum Taq; Invitrogen, Germany) and specific primers for nucleotide (nt) 883 906 and 1288 1265 positions of HDV genome were used [ 30].

Detection of viral nucleic acids using real-time PCR (qPCR) and real-time rt-PCR (rt qPCR) CPV DNA was amplified by qPCR using TaqMan® Gene Expression 2× Master Mix Applied Biosystems CDVCDV and CCoV RNA were amplified by rt-qPCR using the TaqMan® RNA-to-Ct(TM) 1 step kit in a 20 μl reaction with 50 ng of template.

(This substantial difference is expected to be even larger in a setting where an expressed Cre does not require a second, TM-mediated step for activation, as is the case with TM-independent Cre expressed from the Prm promoter, but not with TM-inducible CreER, expressed from the Prp promoter).

The latter stands for the worst case condition, in that, for a given pair {r,m}, the search is halted as soon as a feasible TM is found (step 10 of Table 2), because any other TM with the same pair {r,m} would require a larger amount of transmitted power.

Immunohistochemical staining was performed to detect the NET-1 and Ki67 expressions in the sections using Dako Elivision TM Plus Two-step System PV-60000 kit, Zymed, Co. USA).

PCR conditions were one 5-minute cycle at 95°C, 25 (β-actin) or 30 cycles (target mRNAs) each comprising a 30-second step at 95°C, followed by a 30-second step at primer-specific Tm and a 45-second step at 72°C, and one last cycle of 7 minutes at 72°C.