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Leaves for the TS sample were harvested and cut as for the TM sample, subjected to a 3 h mock enzymatic digestion and then frozen.
The reversibility of the unfolding refolding process was assessed by reheating the Tm sample directly after it had been cooled from the previous temperature scan.
Whereas 5% of the non-significantly changed genes in the Tm sample contain previously identified AUG-initiated uORFs, 14% of genes in the list of ISR-translational targets contain uORFs, indicating a significant enrichment (p < 0.003, chi-squared test with Yates correction).
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All the TM samples were contained heavy metals at different concentrations.
The differences were found statistically significant with a p value <0.05 for all TM samples digested with method C for all elements (Figs. 1 and 2).
The samples were subjected to three different acid digestion methods, as will be explained, to identify the most appropriate digestion method to determine the contents of As, Pb, Cd, Ni, Zn, and Fe in TM samples by AAS.
However, TM samples have complex matrices as they are made from either one herb or a mixture of herbs from any part of a plant such as leaves, roots, seeds, and flowers that might have different chemical properties.
The results obtained from all experiments indicated that method C which represented the mixture of HNO3 HCl had given the highest analyte recovery for As, Cd, Pb, Ni, Zn, and Fe in TM samples.
Whereas the axial Young's modulus of the low-frequency (apical) region of Tecta mutant TM samples was similar to that of wild-type TMs, it significantly decreased in the basal region to a value approaching that found at the apex.
The digestion capacity of hydrochloric-nitric acids HNO3 HCl in a ratio of 1 3 mixture had proven to be the best acid combination suitable for the decomposition of TM samples due to the ability of such mixture to release the metal ions from such complex matrices of herbal materials and subsequently to minimize the noise level during the detection procedure.
TM samples were classified as basal or apical based on the cochlear turn from which they were dissected.
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