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Thus, whether PH or TM procedures alter maternal stress responsivity remains unknown.
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TM oversaw procedures.
One source of error is the first fitting procedure of TM and Tm to transmittance curve.
Transcatheter mitral valve-in-valve/valve-in-ring procedures (TM-VIVoR) are increasing.
Life-saving procedures were TM related in seven patients (6.1%): stroke thrombolysis (n = 6) and limb amputation (n = 1).
Determination of the melting temperature (Tm) used the following procedure: the LNA-modified and the non-modified oligonucleotides were mixed with a single-stranded complementary DNA fragment (0.05 nmol of each) in a final volume of 25 μl of Absolute Blue Sybr ROX buffer 1× (Thermo Scientific).
This latter parameter, calculated from the fitted functions to ascorbate minus dithionite temperature dependences of 1/ TM as described in Experimental Procedures, appears as an additional empirical parameter that conveniently compares the strength of dipolar relaxation; the larger the value of the integrated area under the curve, the stronger the dipolar enhancement of phase relaxation.
TM stops this process.
This window is translated by one base pair and a new Tm is calculated and the procedure is repeated till the end of the sequence.
TM conceptualized and supervised the procedure.
We used our own implementations of the STRUCTAL, TM-align, and LOCK2 alignment procedures to compute optimal residue-residue correspondence (alignment) between the newly superimposed proteins.
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