We recently refined the Tm-assay by using a molecular beacon as an internal control for temperature variations over the heat-block in the thermocycler and an automated analysis program for more precise and unbiased Tm acquisition.
Owing to crop rotation, agricultural land cover is highly dynamic (cereal crops, cultivated pastures, fallow land); thus, both ground reference data from 2011 as well as the various high resolution reference images contain a high probability of being different from the situation on July 13 , 2008(Landsat 5 TM acquisition date).
The distal end of the catheter was connected to an iWorx 214 TM data acquisition system (Ladscrib 2.0 software, iWorx Systems, Inc., Dover, NH, USA) via a pressure transducer for continuous recording.
A standard pulse sequence (recycle delay [RD]-90°- t1-90°- tm-90°-acquisition) was used to acquire one-dimensional (1-D) spectra.
A one-dimensional NMR spectrum was acquired for each of all samples, employing the first increment of NOESY pulse sequence (recycle delay-90°- t1-90°- tm-90°-acquisition) with a spoil gradient for water presaturation.
TM participated in acquisition of data, analysis and interpretation of data, and manuscript writing.
TT, SS, HG, TK, MA, TM: Patient management, acquisition, interpretation of the data, statistics, and review of the final manuscript draft.
In conclusion, our findings strongly indicated that, TM improved thermotolerance acquisition by decreasing basal metabolic rate and muscle injury during thermal stress.
In conclusion, the present study indicated that, TM improved thermotolerance acquisition by decreasing each of basal metabolic rate and muscle atrophy.
TM: conception and design; acquisition of data; drafting and revising the manuscript; DAM: conception and design; acquisition of data; drafting and revising the manuscript.
YY, KF, KK, KN, and TM: Conception, study design, acquisition and interpretation of the data, statistics, drafting, and preparation of the final manuscript version.
