Exact(8)
TM (1 mM) was added to cultures for 1 hr to induce CRE activity.
Thus, the Jmjd6 tm 1 Flv and Jmjd6 tm 1 Ysfk alleles represent double knockouts of the Jmjd6 and 1110005A03Rik genes, while the Jmjd6 tm 1 Gbf allele ablates only Jmjd6 gene expression.
Jmjd6 tm 1 Flv -/- and Jmjd6 tm 1 Ysfk -/- animals show no expression of 1110005A03Rik and Jmjd6, while the expression of Sfrs2 remains unaffected by the gene targeting.
Mice were also i.p. injected with TM (1 mg/kg BW) or vehicle (DMSO).
In contrast, the Jmjd6 tm 1 Gbf allele ablates only the expression of Jmjd6.
Mice were treated with TM (1 mg/kg body weight) or vehicle for 24 hr.
It could well be that loss-of 111005A03Rik function in Jmjd6 tm 1 Flv and Jmjd6 tm 1 Ysfk knockout mice might contribute in addition to the Jmjd6-deficiency to the phenotypic discrepancies observed.
An expression analysis of Jmjd6, 111005A03Rik and Sfsr2 in the three different mouse alleles confirmed that Jmjd6 tm 1 Flv and Jmjd6 tm 1 Ysfk mutant mice are double knockouts of the Jmjd6 and 111005A03Rik genes.
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