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This method provided a linear assay over five to six orders of magnitude and reproducibly detected titres as low as 103 molecules of the target CVYV cDNA.
All three of these individuals were male, and had significant AMA titres, as well as positive ANA of unknown specificity [ 83].
In the present study, it was shown that maternally derived antibodies in serum were able to neutralize PCV2-infection in an in vitro SN assay but could not avoid PCV2-infection of the pig since seroconversion was observed in pigs with maternal SN titres as high as 6 log2.
In the RTS,S/AS02D groups, the 0,1,7-month schedule induced higher month 19 anti-CS titres as compared to the 0,1,2-month schedule (44 EU/mL [95% CI: 33, 58] vs 20 EU/mL [95%CI: 14, 29] respectively) (Figure 3).
Experimental evidence for an up regulation in glucocorticoid titres as a response to stressful conditions in nestlings is mixed ([18], [28] but [27]), even though some studies suggest that altricial nestlings may indeed exhibit adequate physiological and behavioural adjustments in response to stressful situations [29], [30].
Steam exploded wild grass (Achnatherum hymenoides) provided the best ethanol titres as compared to other biomasses.
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AAV-GFP was also produced to initially test for optimum serotype and titre, as well as accuracy of intranuclear injections.
In order to determine the optimum AAV serotype and titre, as well as to confirm the accuracy of intranuclear injections, AAV-2/5,2/7,2/8 AAV-2/5,2/7,2/8P were initiandy produced.
Conjugate B1T1 and B2T1 showed higher serum IgG/IgA titre, respectively, as well as high secretory IgA plus secretory component (Sc) both in lung and intestinal washes.
The supernatant from infected C6/36 cells was collected for viral titre determination as well as for subsequent experimental applications.
The questionnaire was designed to obtain information on variables regarded as potential risk factors for Leptospira seropositivity defined by serological titres ≥ 1: 100 as well as for Leptospira infection defined by serological titres ≥ 1: 400 [ 18].
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