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The detection limit of the test was established by testing in parallel the phage titre increase that occurred in the same A172 cultures (109 CFU/200 µl) spiked with 12, 6, 3 or 1 A170 MSa-sensitive bacteria.
Compliance with EMA criteria (as defined for annual re-licensure trials) for the immunogenicity of influenza vaccines in elderly people for both the HI and SRH methods was also assessed: a GMTR >2, a seroprotection rate ≥ 60%, and a rate of seroconversion or significant titre increase ≥ 30% using either test [ 24].
21 22 Therefore, with two blood samples collected at an interval of at least 16 weeks, we defined IgG seroconversion for amoebiasis as ≥1 64 at the second sampling as well as a fourfold titre increase when compared to first sampling results, which indicates recent infection.
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Violin plots showing median (black square), 25% and 75% quantiles (thick black line) and 95% distribution (in grey) of net titre increases at different time points after infection (n = 1,420).
The α-amylase titre increased significantly in cane-molasses medium (60 U ml−1) as compared to that in the synthetic medium (26 U ml−1).
The titre increased 8 times to 0.66 mM (1.2 % Cmol) when E. coli BW25113 (DE3) Δpta-ΔldhA also expressed gltA and acnA.
To assess the statistical significance of titre increases (ELISA, IFA) after sporozoite challenge we used a Wilcoxon signed-rank test to compare titres before and after sporozoite challenge.
The cultures were then transferred quantitatively on a lawn of the A170 MSa-sensitive bacteria (105 CFU/4 ml soft agar) to determine whether the original phage titre increased.
The NO titre increased slightly at the 9th h post infection and significantly at the 24th h post infection in comparison to the uninfected control (Fig. 4A).
Lipid titre increased rapidly up to 48 h.
In addition, IgG titre increased rapidly after a subsequent boost.
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