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The viruses were titrated by limiting dilution plaque titration on 293A cells, and used at 50 to 100 plaque-forming units/cell.
The protein structure remained practically intact up to the end of the first stage, whereas further titration on the second stage resulted in protein denaturation.
Field parameters pH, temperature (T), electrical conductivity (EC), redox potential (ORP) and dissolved oxygen (DO) of samples were measured by portable equipments (Horiba, Japan), and alkalinity was determined using the acid neutralizing titration on site.
The adsorption tests were preformed using colloidal titration on a range of core or rock samples taken from oil wells in Saskatchewan in an attempt to reveal the structure property relation.
Three sets of ATPγS titrations were performed; 1) ATPγS titration on CaMKII, 2) ATPγS titration on CaMKII with GST-NR2A, and 3) ATPγS titration on CaMKII with GST-NR2B.
Viral titres were determined by virus titration on Madine Darby canine kidney (MDCK) cells.
At indicated time points, supernatant was collected and infectivity titers determined by titration on naïve Huh7 cells.
Supernatant was collected and 10 µl were used for phage titration on isopropyl β-D-1-thiogalactopyranoside/X-Gal (IPTG/X-Gal) (Fermentas) lysogeny broth agar plates.
At the selected time points medium from the TOCs was removed and analysed for progeny virus by plaque titration on CK cells.
Rescued viruses were amplified in BHK cells using DMEM without serum for infection and growth, before purification over sucrose cushions and titration on CEFs.
Viral titres were determined after thawing of the tissue sections followed by homogenization and resuspension by virus titration on MDCK cells.
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