Potentiometric titration of polyelectrolyte samples was performed using a pH meter pH-340 (Econix Express, St .Petersburg, Russia).
The IEC value obtained by titration of three samples (uncertainty 95%, t = 2.92) was 0.48 ± 0.12 meq/g whereas that obtained by Cu2+ exchange was found to be equal to 0.31 ± 0.07 meq/g.
Dosages were determined by estimating protein mass per milliliter of the vaccine candidates at the time of injection and later calculating titers by back titration of the samples.
In this study, we confirm the existence of two conformer pools by different methods not dependent on photoexcitation, namely, absorption spectroscopy and pH titration of dark samples.
RNA titration of M2-1 samples incubated in the presence of 5 mM EDTA also led to an increase in radius of gyration, however, its magnitude was smaller (Rg = 5.7 vs Rg = 8.0 nm in the absence of EDTA).
After log10 titration of serum samples on Vero E6 cells, cytopathic effect was determined at 5 dpi and TCID50 infectious titers were calculated by using the Spearman Kärber method (27, 28 ).
Titration of a sample of [CH3-Met]LD with TMAO results in a loss of intensity of the random coil Met18 resonance, and a parallel increase in the intensity of a broad resonance near the folded position.
The optimization of acid injection was found to be extremely important, because inefficient analyte stacking introduced by insufficient titration of the sample plug as well as an overlong HCl injection led to peak broadening.
