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Briefly, adenovirus expressing Cre recombinase Adeno-Cre thee University of Iowa Gene Transfer Vector Core, Iow, IA) were titrated by Adenoviral Titration Kit (Clontech, CA) using instruction provided by the manufacturer.
The protonation constants of ligands and formation constants of binary complexes was determined by means of a MOLSPIN automatic titration kit (Molspin Ltd ,Newcastle-upon-Tyne). A Hamilton Bonaduz AG microsyringe for 250 μL was used with the auto burette.
Virus titers were assessed with the Lenti-X qRT-PCR titration kit (Qiagen,Valencia, CA).
The virus pellet was resuspended, and the virus titer was measured using the Lenti-X qRT-PCR Titration Kit (Clontech).
The virus titer was determined with a Fast Plax titration kit according to the manufacturer's instructions (Novagen, Madison, WI).
The M.O.I. used for Ad-CMVZGP or Ad-CAGoptZGP on HEK 293 cells was based total infectious particles per ml as determined by immunocytochemistry against the hexon protein (Adeno-X rapid titration kit, Clonetech, Mountain View, CA).
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At the back, a team of scientists in lab coats are busy with titration kits and molecular formulas, while Michelin-starred chefs are cooking up some scrambled eggs – without cracking any eggs.
In the titration period, patients were issued an electronic diary and a dose-titration kit containing five doses of each of the five dose strengths (200, 400, 600, 800, and 1200 μg) of FBSF.
After purification, the titration of each recombinant adenovirus was determined by an ELISA titrating kit.
The alkalinity was determined directly in the field using a titration quick test kit (Merck, 1.11109.0001).
Based on field and laboratory investigation using rapid test kit and titration technique, it was observed that malpractice was widespread in the iodized salt business.
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