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However, in more concentrated 1H NMR titration condition (2 mM), 12+ and 22+ decomposed to form the product one of their amide arm is eliminated.
Therefore, anions with weak basicity such as chloride, bromide, and hydrogen sulfate bound to the receptors 12+ and 22+ only through hydrogen bonds while anions with strong basicity such as fluoride, acetate and dihydrogen phosphate bound to the receptors 12+ and 22+ only through charge transfer interactions at UV vis titration condition (20 μM).
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The MAQC results showed relatively low technical variability in the intra-site and inter-site measurements, and high inter-platforms concordance for the thousands of genes identified as differentially expressed between 2 reference RNA samples explored under 4 titration conditions [ 10, 11].
The Cd2+-titration conditions were carefully defined to prevent Cd2+-induced structural changes in SLVI and/or RNA aggregation that may result from an excess of Cd2+ ions.
A titration of conditioned media (no CAT-354) was performed using doubling dilutions.
Based on the checkerboard titration result, the condition of ELISA for the detection of canine NGAL was optimized as 1 3000 diluted mouse serum and 1 800 diluted rabbit serum, which were used as the detector antibody and the capture antibody, respectively.
Titration to alkaline conditions was possible, but after the isoelectric point was passed at a pH of ~10 the zeta potential did not fall below the critical −30 mV.
However, binding of glycerol to Gal3C is insignificant in solution at room temperature, as monitored by NMR spectroscopy or isothermal titration calorimetry under conditions where lactose binding is readily detected.
The conditions of PCR were determined by cycle titration to avoid saturating conditions.
The detection limits of the microarray were determined under these conditions by titration of chromosomal DNA and unlabelled amplicons followed by hybridisation to determine the levels of sensitivity that could be obtained with the microarray.
This concentration was determined by titration to reach optimal conditions for sensitivity and specificity.
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