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IV was evaluated by titration by using the EN 14111 2003 standard.
The physicians and patients were kept blinded to the treatment allocation until the extension phase completion by blinded titration by using a similar placebo.
The absorbed sulfur trioxide (sulfuric acid) is determined by an absorption titration by using barium chloride as the titrant and thorin as the indicator.
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Optimal work concentration for the conjugates was determined by chessboard titrations by using an RF-positive serum pool.
The vaporized sample was then transferred into the titration cell by using dry nitrogen gas with a flow rate of 300 ml/min.
The protonation/deprotonation constants were calculated from the titration data by using the program HyperQuad 2000 version 2.1 (Hyper Quad Solutions.
Analysis of the UV/Vis titration data by using Specfit 35 software revealed a 1 1 stoichiometric association constant of log K=3.38±0.04 (K=2396 M−1), which is in good agreement with the value determined by H NMR spectroscopy.
The formation constant and thermodynamic parameters for the inclusion of Arginine in ArgRS were measured by the titration calorimetry method by using an ITC MicroCal 200 (GE Life Sciences).
pH titrations were performed by using a series of buffers.
The titration data were analyzed by using the sequential binding model available in the Origin software provided by MicroCal, which yielded the following parameters associated with the bisANS/PDC-109 interaction: equilibrium association constant (Ka), enthalpy (ΔH), entropy (ΔS) and the stoichiometry of binding (n, which corresponds to the number of bisANS molecules bound per molecule of PDC-109).
Titrations were done manually by using the microinjectors.
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