Sentence examples for titrating the amount from inspiring English sources

Optimum inducible protease activity for each of the cis-sTEVp construct was determined by titrating the amount of free sTEVp construct against a fixed amount (300 ng) of the C34V-fused construct.

This was to avoid the possibility of titrating the amount of Gal4 protein available for the UAS vectors.

In addition, the authors may consider titrating the amount of LC3 on the membrane and quantifying the dissociation rate of oligomerized p62 from the membranes coated with different concentrations of LC3 proteins.

By titrating the amount of RNA in the NMR experiment, we estimate the binding affinity of this zinc finger to the polyA15 RNA to be 60 100 μM (Supplementary Fig S4).

At maximum expression levels, both wild-type and variant GREM2 efficiently inhibited BMP signaling; however, by titrating the amount of transfected plasmids, we found that at lower concentrations the Q76E variant was at least twofold more potent at antagonizing BMP than wild-type GREM2 (Fig. 1D).

By titrating the amount of tamoxifen to maximize recombination while minimizing animal lethality, we determined that 30 μg tamoxifen/g body weight/day injected on three consecutive days is the optimal condition for the αMHC-MerCreMer system to induce recombination in the Rosa26-lacZ strain.

Unlike 'rescue' experiments in mammalian cells treated with siRNA, one can carefully titrate the amount of synthetic mRNA microinjected into zebrafish embryos.

To test if this reduction of Eg5 on spindle microtubules is sufficient to explain the failure of spindle formation, we titrated the amount of wild-type Eg5-GFP in depleted extract and measured both the percentage of spindle recovery and the amount of Eg5-GFP in spindle structures, a quantitative experiment for which the Xenopus egg extract system is well suited.

A tight binding inhibitor (pyrrolidine-containing DNA) was used to titrate the amount of active AlkA.

We titrated the amount of lectins used in this blocking assay, while holding the biotin-CTB concentration constant.

To examine in more details the kinetics of WRN-mediated processing of telomeric substrates with a 3' overhang, we titrated the amount of WRN added to the exonuclease reactions.