Sentence examples for titrated concentrations from inspiring English sources

We thus added titrated concentrations of IL-2 (range 12,5 U/ml to 200 U/ml) with a fixed concentration of IL-15 (10 ng/ml) to Treg cultures to determine the optimal IL-2 dose in our system.

For activating 2C transgenic CD8+ T cells, the cells were incubated with mitomycin C-treated (inhibition of DNA synthesis) [27], [28], [29] LPS-stimulated B blasts [42], [43] from B10.A mice and QL9 peptide (QLSPFPFDL, Proimmune Inc., Oxford, United Kingdom) in titrated concentrations.

To characterize antisense-mediated transcriptional repression in TX-TL, we first titrated concentrations of the pT181 attenuator (Att-1) reporter plasmid to determine an appropriate level of SFGFP output for our experimental setup.

For competition studies between anti-ADAMTS-5 antibodies, 2B9 was amine-coupled to a CM5 chip (∼300 RU) and titrated concentrations of ADAMTS-5 in the presence of 200 nM of competitor antibody were injected.

As in Figure 4A, we found inverse proportionality between S l o p e X t a l k and titrated concentrations of antigenic peptide, which established a calibration curve to back-calculate fold changes in the antigenic capacity of tumor tissue suspensions.

Titrated concentrations of IgG1 and IgG2a myeloma proteins were used to generate a standard curve, and the IgGs were detected with the same labeled rabbit anti-mouse IgG1 or IgG2a antiserum.

Oligomycin addition can be followed by a carefully titrated concentration of a protonophore, such as FCCP (carbonyl cyanide p-trifluoromethoxyphenylhydrazone), to give uncoupled respiration ('state 3FCCP' or 'state 3u').

Titrating concentrations of GlpQ protein and secondary antibody were tested in a checkerboard assay to determine the optimal concentrations for detecting B. miyamotoi sensu lato antibody.

To determine suboptimal concentrations of NE, we titrated NE concentrations by measuring energy expenditure as total oxygen consumption rates (OCR).

In this study, we titrated the concentrations of LFnBLA and PA in preliminary experiments and chose the lowest concentrations that consistently produced high FRET disruption.

Since BH3I-1 contanns aromatictic ring, J surface map could be calculated at all titrated ligand concentrations (Figure 7B & C).