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In certain experiments RU486 concentrations were titrated as indicated.
Mifepristone (RU486) (Sigma) was fed to adult flies at a final concentration of 160ug/ml in the food media as previously described [ 3], and in certain experiments mifepristone concentrations were titrated as indicated.
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Fresh virion samples were prepared, either with or without 1 mM IPTG induction, PEG precipitated and titrated as described.
This vector was constructed, produced, purified and titrated as described elsewhere.
In LPS-treated piglets, tachypnea is commonly seen with the acute phase response and requires no treatment End-tidal carbon dioxide (mmHg) 35 45 Titrate ventilator support as indicated Core tempature (°C) 101 103 Hypothermia can be avoided by housing piglets in temperature-controlled cages and by maintaining active warming during surgery.
Lentiviruses were produced and titrated as described [33].
Cell supernatants were titrated as serial 10-fold dilutions on Vero cells as previously described [36].
Altering the GO term distance threshold also had the anticipated result where the stringency of predictions was titrated as expected.
Stocks of the rescued viruses were grown in BTY cells in 25 cm2 flasks and were titrated as described below.
Lentiviral vectors were titrated as described [ 74].
The infectivity of virus solution was titrated as previously described (Iwami et al., 2012a).
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