Sentence examples for titrated amount from inspiring English sources
After washing in demi-water and phosphate-buffered saline, the appropriate titrated amount of antibody was added and incubated for 60 minutes at room temperature.
After 2-3 hours they can be replica plated onto plates lacking the appropriate amino acids and the titrated amount of AT (master plate), and at the same time onto plates covered with a filter membrane.
Briefly, after 5 min incubation with Fc receptor block (Miltenyi, Bergisch Gladbach, Germany) cells were stained with a titrated amount of antibodies for 10 min in the dark at room temperature before being washed and immediately analyzed on a LSRFortessa cytometer (BD Biosciences, Heidelberg, Germany).
We next used titrated amounts of the blocking mAb, while keeping the peptide concentration constant (Fig. 4B).
For analysis of surface phenotype after proliferation, cells were washed once with PBS and incubated with previously titrated amounts of antibodies to CD4, CD14, CD16, CD19, CD8, CD45RA, CCR7 and CD25 for 30 min at 4°C.
To this end, titrated amounts of purified M-860 or mIgG1 were added to wells precoated with human, bovine, or murine LTF.
Titrated amounts of Carraguard were tested against sub-saturating doses of RT-SHIV vs HIVBal and HIVMN (Fig. 1B).
To test if this reduction of Eg5 on spindle microtubules is sufficient to explain the failure of spindle formation, we titrated the amount of wild-type Eg5-GFP in depleted extract and measured both the percentage of spindle recovery and the amount of Eg5-GFP in spindle structures, a quantitative experiment for which the Xenopus egg extract system is well suited.
We titrated the amount of lectins used in this blocking assay, while holding the biotin-CTB concentration constant.
To examine in more details the kinetics of WRN-mediated processing of telomeric substrates with a 3' overhang, we titrated the amount of WRN added to the exonuclease reactions.
We titrated the amount of starting cDNA and determined the number of amplification cycles to obtain reproducible quantitative performance of the RT PCR assay for MRP-1/CD9, KAI1/CD82 and CD151.
