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Fat tissues were fixed in universal fixative.
Tissues were fixed in 10% buffered formalin and paraffin embedded.
Tissues were fixed in 1% glutaraldehyde, 4% paraformaldehyde in PBS and washed.
Tissues were fixed in 10% buffered formalin and embedded in paraffin.
For histological processing, liver tissues were fixed in 4% formalin solution.
The tissues were fixed in 4% paraformaldehyde and processed for immunohistochemistry to detect Ki67 (1 200, bioworld).
Liver tissues were fixed in 10% buffered formalin, embedded in paraffin and stained with haematoxylin and eosin.
Resected teratoma tissues were fixed in 10% formalin, embedded in paraffin blocks, sectioned at a thickness of 5 μm, and mounted on glass slides.
For hematoxylin and eosin staining (H&E staining), liver tissues were fixed in 10% neutral buffered formalin and embedded in paraffin.
Briefly, tissues were fixed in 4% neutral formalin for 24 h and embedded in paraffin to be cut into slices, which were stained by hematoxylin and eosin.
Left lobe of livers and epididymal adipose tissues were fixed in 10% neutral buffered formalin for more than 24 h, and they were embedded in paraffin block.
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