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The human nasal tissues utilized in our study represent patients with varied degree of olfactory dysfunction (Table 1).
All tissues utilized in this study have received institutional REB approval.
Sepal and petal tissues utilized in reverse transcription PCR analysis were hand-dissected from ag inflorescences.
Tissues utilized in these experiments included repair tissue and grossly normal articular cartilage from within the same joint.
To be able to compare RNase Dre1 and RNase Dre2 expression with that of RNase A genes, we used the same tissues utilized in the analysis by Cho and Zhang [ 16].
The range of genes is also similar to the GOSlim classes identified and already described by the shotgun approach adopted by Kaur et al. [ 15] and is unsurprising considering the range of plant tissues utilized in the two studies is similar.
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Analysis for systemic infection was performed on tissues harvested from infected mice or on cells isolated from the indicated tissues utilizing in situ hybridization, real time PCR analysis and co-culture with PHA activated allogeneic human PBMC as previously described [22], [24], [26].
Floral tissues utilized within the in situ hybridization validation were derived from Arabidopsis thaliana of the Landsberg erecta ecotype.
Localization of anti-ERβ antibodies in formalin-fixed, paraffin embedded disc specimens required antigen retrieval techniques; both cells and embedded tissues utilized an overnight incubation in primary antibody.
The presence of virus antigens in the fish tissues utilized three different types of assays: immunohistochemistry, immunochromatography, and antigen ELISA.
The PtGen2 microarray contains 25 848 (26 496 total features minus buffer blanks and duplicate spots) amplimers of cDNA clones derived from thirty-six cDNA libraries constructed exclusively from loblolly pine (P. taeda) root and needle tissue; no embryonic tissue was utilized in its construction [ 7, 18].
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