Blood was collected and tissues of interest were removed.
And the porosity requirements vary among the cells and tissues of interest (Thankam and Muthu 2014c).
Briefly, prior to elemental analysis, the tissues of interest were digested in nitric acid (ultrapure grade) overnight.
The tissues of interest (0.2 to 0.5 g) were homogenized in 1 mL of acetonitrile-water mixture (1 1).
For the biodistribution study, the tissues of interest and blood were then weighed and counted, and data are expressed as percent injected dose per gram of tissue.
The organs and tissues of interest were rapidly excised, weighed, and the radioactivity was determined using the automatic gamma counter (see above).
Tissues of interest are the same as used in Figs. 3, 4, and 5, i.e., the muscle, fat, and cortical bone.
Images were acquired with a microSPECT/CT camera at various time intervals and reconstructed to allow activity quantification in the tissues of interest.
However, since renal clearance is downstream of delivery to other tissues of interest (e.g. tumour), no significant effect is expected on these.
Tissues of interest were removed and weighed, and radioactivity counts were determined with an auto well gamma counter and corrected for background radiation.
Antibody variants that differ in antigen binding sites and size have been generated and evaluated as imaging probes to target tissues of interest.
