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We then called tDMPs for each tissue between the tissue of interest and all the other tissues (Materials and Methods).
Expression of Brk protein was confirmed by both Western blotting of purified mammary epithelial cells (MEC and IHCC analysis of formalin fixed paraffin embedded (FFPE) mammary tissues (Materials and methods).
For meaningful comparisons across markers and patient groups, we adopted the concept of hypermethylation to describe increased methylation in tumor relative to normal DNA, and determined gene-specific thresholds based on Dm values in the respective normal reference tissues (Materials and Methods, Suppl. Table 4).
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A paradoxical type of bleeding is found when tissue materials (as the consequence of shock, trauma, burns) enter the bloodstream and cause clotting within the vessels.
Tissue materials were genotyped on the Affymetrix 6.0 array, according to the manufacturer's protocol.
Tissue materials were obtained from pretreatment biopsies.
Parallel proteomics investigations showed over 800 protein spots in the gels from tissue materials.
Tissue materials used for RNA isolation differed from the cohort used for immunohistochemistry.
BB carried out the surgical removal of the sample tissues and GE, the pathological assessment of the surgical tissue materials.
The advantages of time-resolved luminescence detection for microscopy studies, especially for highly autofluorescent tissue materials, are obvious.
All samples were obtained with informed consent and the study of the tissue materials was approved by the Local Ethics Committee.
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