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Biopsy samples of NPC and adjacent normal nasopharynx tissue were obtained from patients undergoing surgery.
Human astrocytes isolated from human cerebellar tissue were obtained from ScienCell Research Laboratories.
Frozen and fixed adult human hypothalamus and pituitary tissue were obtained from Harvard Brain Tissue Resource Center (Belmont, MA).
Glioma biopsies of fresh tumor tissue were obtained from patients operated at the Clinic of Neurosurgery, Lund University Hospital, Sweden.
Paired samples of omental and subcutaneous adipose tissue were obtained from bariatric patients that underwent weight-reduction surgery and signed informed consent forms [23].
The intrapair correlation coefficients for each tissue were obtained from normalized expression data and ranged from 0.96 and 0.99 for EAT and SAT; and from 0.94 and 0.98 for MAT (Figure S1).
Biopsies of human abdominal subcutaneous adipose tissue were obtained from surgical operations for nonsystemic abdominal disorders (mainly uterine extirpation, gall bladder disease) from seven otherwise healthy and nonobese females (BMI 26.1±1.8 kg/m26.1±1.8
For Western blot analysis, 9 head and neck cancer tissues and 1 normal oral mucosal tissue were obtained from patients underwent surgery at Dental hospital, Peradeniya, Sri Lanka after their written informed consent.
Paired samples of visceral and subcutaneous adipose tissue were obtained from a subgroup of 190 Caucasian men (N = 91) and women (N = 99), who underwent open abdominal surgery (described in detail elsewhere) [19].
A single mesh section (3 × 4 cm) and adhesive tissue were obtained from each explanted mesh.
HASMCs originating from normal human tissue were obtained from Bio-Whittaker (Walkersville, MD, USA).
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