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The SUV was calculated as the ratio of radioactivity in each ROI (MBq) per gram of organ tissue (weighed post-mortem) and radioactivity in the whole-body ROI (MBq) per whole-body weight (excluding tail).
Mouse tissues or cells were snap frozen in liquid nitrogen, ground fine in a pestle and mortar (only for tissue), weighed and lysed in the following buffer (150 mM NaCl, 50 mM Tris, 1% NP-40, 0.1% SDS, 0.5% deoxycholate, 5 mM EDTA, 1% Triton X-100, pH 8.0) with protein inhibitor cocktail (Complete Mini, Roche) to a standard final concentration.
Three hours after injection, the tumors were isolated from the surrounding tissue, weighed, and placed in 0.5 ml formamide.
Organs were excised, washed with saline, dried with absorbent tissue, weighed, and counted on a gamma-counter.
At 1, 5, 7 and 10 days, discs were removed, blot dried with tissue, weighed and then placed in fresh medium.
Ten-week-old mice were euthanized in a CO2 chamber; both tibias were removed, dissected free of surrounding soft tissue, weighed and immediately frozen.
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Using a scalpel and forceps, and working under the safety cabinet, a cube of tissue weighing approximately 1.5 g is dissected from the samples provided.
To calculate the effective dose, the individual organ dose values (equivalent doses) are multiplied by the respective dimensionless tissue weighing factor.
Liver tissues (weighed ∼100 mg) were harvested and homogenized in chloroform/methanol (2 1 v/v) using a Polytron tissue grinder (Kinematica AG, Luzern, Switzerland).
Seven days after treatment, the uteri were removed from these animals, stripped of extraneous tissues, weighed, and chilled in ice-cold saline before analysis.
The researchers collected the subjects' used facial tissues, weighing them to assess their cold symptoms.
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