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Forearm blood flow (FBF), in ml/min/100 ml tissue, was measured using VOP (Hokanson, EC6, D.E., Bellevue, WA, USA) described elsewhere [ 9] in the non-dominant forearm, kept above the heart level, with a mercury-in-silastic strain gauge [ 10] placed on the upper third of the forearm at its maximum circumference.
MPO activity on the homogenized colon tissue was measured using the same technique as described above.
Tumor burden for each individual tissue was measured using PCR and q-RT-PCR incorporating Taqman chemistry.
The hydroxyproline (HYP) content of the lung tissue was measured using an ELISAs.
The fluorochrome concentration inside the tumour tissue was measured using volume of interest analysis.
The area of the damaged tissue was measured using Image-j®image processing and analysis software (Research Services Branch, NIH).
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Levels of myeloperoxidase (MPO) in colonic tissue were measured using an ELISA kit (Cell Sciences, Canton, Massachusetts, USA).
TL and pyruvate in subcutaneous tissue were measured using microdialysis.
Histones H3 and H4 levels in plasma and lung tissue were measured using ELISA kits (Active Motif Inc. Uscn Life Science Incc).
Levels of MPO in colonic tissue were measured using an ELISA kit (Cell Sciences, Canton, Massachusetts, USA).
BMP-7 levels in cartilage tissue were measured using sandwich enzyme-linked immunosorbent assay.
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