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The fixed tissue was frozen in Tissue Tek OCT compound (Sakura) with liquid nitrogen.
Tissue was frozen in liquid nitrogen and frozen sections of 5 μ were cut.
The tissue was frozen shortly after the patients died.
The tissue was frozen and kept in storage in Leeds where the operation had taken place.
Scientists were not sure if this maturation process would continue normally after being paused for more than a decade while the tissue was frozen.
Tissue was frozen in OCT (Tissue-Tek) for cryo-sectioning.
The tissue was frozen, and 20-µm cryostat sections were taken (HM430, Microm, Germany).
The tissue was frozen, cut into serial 100 µm thick sections and stained with neutral red.
Lymph node tissue was frozen in liquid nitrogen and homogenized using a mortar and pistil.
Tissue was frozen in a Histo Bath (Shandon/Lipshaw) and stored at −80°C.
Therefore, the placenta tissue was frozen immediately in liquid nitrogen, embedded in OCT and stored at −80°C until use.
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