For some large decapod species, only tissue (legs or abdominal muscle) was obtained for barcoding and the samples were stored as tissue vouchers, accompanied by photographs taken prior to DNA extraction.
After collecting tissues, vouchers should be prepared either as study skins (most birds and mammals) or fixed in a 3.7x buffered formaldehyde solution and then transferred into 70% ethanol for long-term storage.
Tissue and voucher specimens (whole fish or head) were collected for every specimen and stored in ethanol.
All tissues and voucher specimens were deposited in the Sección Genética Evolutiva, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.
All tissues and vouchers should be lodged and cataloged within a recognized research collection, along with all necessary permits (collection, import, export, Convention on International Trade in Endangered Species of Wild Fauna and Flora, Animal and Plant Health Inspection Service, etc).
In total, 50 100 mg of infected plant tissue from herbarium voucher specimens (Table 2) were taken and disrupted in a mixer mill (MM2, Retsch, Germany), using six iron balls of 3 mm diameter per sample.
All pollinator sequences (Additional file 5) were sequenced de novo from recent tissue collections with voucher specimens deposited at the Iziko South African Museum.
The integration of (usually) silica-dried leaf tissue with herbarium vouchers and their associated metadata makes the collection a valuable resource for a wide range of collections-based research involving ferns.
BB and SW contributed equally to the manuscript, specified criteria for a model system, provided herbarium material, tissue, and DNA vouchers, generated capillary sequence data and drafted the manuscript.
Finally, it is necessary to deposit all generated sequences with an open access repository, such as GenBank, and designate the voucher or tissue holding institution along with any publications emanating from the use of tissues to ensure fidelity and linking of all data to the original source organism and allow attribution of the collection.
We collected full voucher specimens, tissue samples, photographs, and microhabitat information.
