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Replicate arrays for tissue survey data were averaged.
We repeated the analyses in an independent mouse tissue survey [9] and detected the same result (Figure S2).
Human gene expression data was obtained from the Novartis human tissue survey [20] processed with the gcRMA preprocessing probe set algorithm and consists of 79 different tissue samples.
We examined lymphocyte gene expression data obtained on a sample of 1240 individuals [10], and we detected short-range coexpression clustering as seen in the tissue survey data.
†Data from separate tissue survey of 2000 tonsils (July 2000-August 2002) in southeast England (including London) not included.
Expression profiles of the genes encoding these proteins were obtained from mouse C2C12 cells undergoing myogenesis in vitro, as well as a mouse tissue survey dataset.
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Previous studies of coexpression have focused primarily on human tissue surveys [3].
In two tissue surveys, one of 61 tissues in mouse and another of 73 tissues in human [4], we computed measures of coexpression for all pairs of genes over a range of intergenic distances (see Methods).
We assembled a large collection of microarray data, including tissue surveys, genetic mapping studies, small-molecular perturbation of cell lines, and comparisons of diseased and normal tissues, generated using several microarray platforms (Table 1; Table S1).
Continuing to archive and test tonsil specimens, especially in older birth cohorts, and other complementary large scale anonymous tissue surveys, particularly of post-mortem tissues, will further refine the calculated prevalence of PrPCJD.
The SEM systems of 3D scanning therefore fit between TEM and light microscopy in resolution but with a far greater volume of tissue surveyed with the advantage of greatly reduced working man-hours.
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