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One side of the tissue strip was attached to an isometric transducer that records changes in tension.
Subsequently, a tissue strip from the right ventricle was dissected and used for CV measurements as previously described [ 30].
Healthy-looking, well-loaded cells were chosen for analysis, with up to 16 individual cells analysed per tissue strip.
The tissue strip was mounted on the tester using modified 6-cm straighaemostaticticlampspShanghaihai Medical Instruments Groupp) Ltd., Corp. China).
CV was measured with two microelectrodes (Platinium/Iridium (PI20030.5B10), Micro Probe Inc., Gaithersburg, USA) placed on the longitudinal axis of the tissue strip.
In short, the tissue strip was superfused with oxygenated Tyrode's buffer at a rate of 2 mL/min and the temperature was maintained at 37°C.
Similar(48)
This minimally invasive technique provides less soft tissue stripping than the plating technique and results in good biological fracture healing.
The open surgical approach that we used required more soft tissue stripping which made stable fixation mandatory.
The technique is relatively simple to perform and enhances fracture healing by providing proper stability with minimal soft tissue stripping.
Power saw osteotomies require a relatively large open exposure, and thus have disadvantages of soft tissue stripping around the osteotomy site.
The large wound size and extensive soft tissue stripping required for plate fixation and removal are disadvantages of plating techniques [1, 2].
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