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Low risk categories: N1: Breast composed almost completely of fat, with perhaps just a few fibrous connective tissue strands.
Engineering tissue strands such as establishing their culture conditions is highly critical for bioprinting, while immature tissue strands could not be bioprinted successfully due to significant decomposition in their structure during extrusion (Supplementary Fig. 6).
Figure 3: Mechanical characterization of tissue strands.
Figure 2: Fabrication of tissue strands.
Figure 4: Functional and transcriptional characterization of tissue strands.
Middle: Tissue strands were self-assembled into a "T" shape.
To further validate the potential of tissue strands for fabricating functional cartilage tissue, we examined the functionality of cultured cartilage tissue strands.
To test the potential of tissue strands for self-assembling into a larger tissue patch, fusion experiments were carried out between matured cartilage tissue strands.
Before bioprinting, tissue strands were loaded into the detachable nozzle under sterile conditions.
Tissue strands with appropriate mechanical properties were used for the bioprinting study.
In order to bioprint tissue strands, we designed and customized a detachable nozzle system.
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