A schematic diagram showing (left) the excised specimen, (centre) a ~5-mm-thick tissue slice and (right) a 5-μm-thick tissue slice that has been stained.
Each sectioned tissue slice was mounted directly to a non-fluorescent PET microscope steel frame slide (76 mm × 26 mm, 1.4 μm thick, Leica Microsystems, Germany).
One or more subsections of every 5-mm-thick tissue slice that included tumour and fiducial markers were cut and embedded in paraffin blocks followed by microtome cutting.
(A, C) Pt 1. (B, D) Pt 2. (A) and (C) show the same tissue slice, with radiography in (A) and a photo scan in (C).
To analyze the spatiotemporal dynamics of network activity in a brain tissue slice, it is useful to record simultaneously from multiple locations.
The numerical results are well consistent with tissue slice experiments and moreover, our model predicts more corticomedullary osmolality gradient in outer medulla than previous influential 1-D simulations.
Traditionally, rat hippocampal tissue slice models are used as an in vitro electrophysiology assay to assess seizurogenic potential in early drug development despite non-clinical species-specific differences noted during in vivo seizure studies.
However, these devices are not well suited for recordings from acute brain slice preparations due to a dead cell layer at the tissue slice border that appears during the cutting procedure.
To overcome this problem, we propose the use of protruding 3D electrodes, i.e. tip-shaped electrodes, allowing tissue penetration in order to get closer to living neurons in the tissue slice.
Therefore we anticipate small variation in MALDI signal response across the tissue slice.
Panel A shows the taste bud containing tissue slice in Nomarski optics, while panel B shows the same section under fluorescence.
