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Artifacts such as tissue shrinking during TEM sample processing are always a concern in such quantitative studies.
Most problems with biodegradable OM are related to the duration of the degradation process with a consecutive change of the local tissue environment caused by foreign body reaction and tissue shrinking mainly within weeks, but also up to many months after implantation.
Additionally, the long-term storage of (type) specimens in desiccating preservatives such as ethanol results in changes to specimen colour and tissue shrinking, which makes morphological comparison difficult.
Since tissue shrinking was still acceptable and Mib-1 was easily detected, we shifted to the use of Neo-Fix and used it for more than 6 years, but due to commercial problems this fixative was no longer commercially available in Italy at the end of 2003.
Similar(56)
In the course of maturation, however, the scar tissue shrank and lost water, resulting in negative PE values by day 56 (white arrows).
During radiation therapy, parotid glands, the tumor, and surrounding tissue shrink, deform, and shift to the body midline, leading to variations of the actual parotid dose [ 12, 19].
He provides simple explanations of common problems with easy fixes: pork chops tend to twist and buckle during cooking because the fat and lean tissues shrink at different rates; scoring the fat will help.
Eventually, the tissue shrinks, trailing a parched shadow.
And while the fat tissue shrinks, nothing else appears to -- not the brain, the heart, the liver, or any other organ.
The underlying connective tissue shrinks and brings wound margins close together.
When this tissue shrinks and shortens, it can cause indentations of the skin, and when the fat tissue then underneath is trying to protrude through it, that gives the cobblestone appearance".
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