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In the future work, the construction of more complicated tissue shapes, i.e., T-shape and O-shape, will be included in order to show the variety applications of this device in tissue engineering.
At the macroscopic level, cell and tissue shapes are regulated by surface tension-like properties (Box 1) [18–23].
Additionally, complex morphogenetic processes are also required to sculpt the resulting cells and generate the final tissue shapes and structures required for organ function.
We find that the wing tissue shapes itself through patterned contractions and shear that occur in the context of patterned extra-cellular matrix (ECM) connections to a surrounding cuticular scaffold.
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At present, most tissue constructions rely on encouraging cells to divide and grow on a scaffold to give the tissue shape.Instead, the company grows flat sheets of fibroblast cells (the basic building-blocks of most connective tissues), and then simply rolls the sheet into a tube around a steel rod.
These approaches provide an engineering framework to reproducibly control tissue shape.
This control leads to changes in tissue shape that in turn can affect the tissue's evolution.
Changes in the cytoskeletal architecture underpin the dynamic changes in tissue shape that occur during development [1 4].
Embryonic development involves global changes in tissue shape and architecture that are driven by cell shape changes and rearrangements within cohesive cell sheets.
Our technology for fabricating 3D multilayer hepatic lobule-like, biofunctional tissue enables the precise control of tissue shape in three dimensions.
However, the precise control of tissue shape at the microscale cannot be achieved with various commonly used 3D tissue-engineered building units, such as spheroids.
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