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At present, most tissue constructions rely on encouraging cells to divide and grow on a scaffold to give the tissue shape.Instead, the company grows flat sheets of fibroblast cells (the basic building-blocks of most connective tissues), and then simply rolls the sheet into a tube around a steel rod.
This control leads to changes in tissue shape that in turn can affect the tissue's evolution.
These approaches provide an engineering framework to reproducibly control tissue shape.
Changes in the cytoskeletal architecture underpin the dynamic changes in tissue shape that occur during development [1 4].
Our technology for fabricating 3D multilayer hepatic lobule-like, biofunctional tissue enables the precise control of tissue shape in three dimensions.
This work highlights how regulated protein secretion can synergize with tissue movement to build a polarized BM architecture that controls tissue shape.
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In the future work, the construction of more complicated tissue shapes, i.e., T-shape and O-shape, will be included in order to show the variety applications of this device in tissue engineering.
Previous findings on the shoot meristem suggest that MT organization depends on tissue shape-derived stresses (Hamant et al., 2008).
The complex morphology of pavement cells is a system of choice to decipher the contribution of cell and tissue shape-derived mechanical stresses in MT behavior.
Based on our results, we propose that cells sense mechanical stresses at the subcellular scale, and that they are hence able to integrate cell shape-derived stresses and tissue shape-derived stresses, with a single mechanism.
At the macroscopic level, cell and tissue shapes are regulated by surface tension-like properties (Box 1) [18–23].
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