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Half a millilitre of PBS was added to the well and the cells were scraped off with a tissue scraper and transferred to a 1.5 ml Eppendorf tube.
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Cells were detached with a tissue culture cell scraper (Fisher, 08-100-240) and mechanicalysedysed by passing through a 27½ gauge needle.
Tissue culture flasks and cell scrapers were obtained from Corning and BD Falcon, respectively.
hESC colonies were scraped from the tissue culture plastic using a cell scraper (Sarstedt, cat# 831830), and the resulting suspension gently pipetted to generate a distribution of clumps of the desired size.
Colonies of hESC in feeder-free conditions were removed from the tissue culture plates using a sterile cell scraper and partially dissociated by gentle pipetting.
Using firm pressure, carefully scrape the fat and connective tissue away from the skin using the scraper.
The Laemmli buffer was added directly to the infected cells and scraped off the tissue culture treated Petri dishes using a cell scraper.
Briefly, RLT buffer plus 2-mercaptoethanol was used to lyse cells and a rubber cell scraper was used to release them from the tissue culture plastic according to the manufacturer's instructions.
The Birmingham bone scraper A brand new medical device to remove dead tissue which can become infected from bones has been invented by Dr Deepa Bose, a consultant orthopaedic surgeon based at the University Hospitals Birmingham (UHB) NHS Foundation Trust, and his colleague Simon Williams, a fellow clinician at UHB.
Sheets of cells were washed and scraped off the dish using a cell scraper, cryoprotected in 30% sucrose in PBS and rapidly frozen in OCT (Tissue Tec ®).
Scrapers are also among the most commonly found Neanderthal artifacts, which they used to scrape unwanted tissue off animal skins.
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