Exact(7)
We illustrate the system using a case study that involves searching for blood vessels in an optical tissue scan, and automatically instructing the microscope to rescan these vessels at higher resolution.
Relative expression levels of PTPN4 in different tissues of wild-type mice were determined by quantitative PCR of cDNA samples from a Tissue Scan RT kit (Origene).
In parallel, a total body tissue scan of both MICA and MICB transcription clearly shows that despite original presumptions, and with the exception of the central nervous system, both genes are widely transcribed and therefore possibly translated and membrane-bound.
Furthermore, using Tissue Scan lung cancer array HLRT504 (Origene), we performed quantitative real-time PCR on cDNA obtained from 48 tissues.
Relative levels of mRNA of genes selected for validation were quantified using Tissue scan panel HLRT-02 which includes 24 lung cancer cDNA samples matched in pairs with surrounding lung (normal) cDNA samples.
Tissue Scan panels (8 common cancers [ CSRT101], kidney cancer [ HKRT101], ovarian cancer [ HORT101 and HORT102], and breast cancer [ BCRT101]) and the accompanying pathology and tumor staging reports are available from OriGene (Rockville, MD).
Similar(53)
A wide range of scans were performed, including pelvic, right upper quadrant, cardiac, thoracic, and soft tissue scans.
HCS was firstly applied to MRI data to improve resolution and visual interpretation of regions within the tumour tissue scans.
Aliquots (20 μl) of the incubation solution were spotted onto polyethylene-backed absorbent paper (BenchGuard™, Lennox, Dublin, Ireland), allowed to dry, scanned, and digitized in the phosphorimager parallel with the tissue scans.
Digital microscopy is not new - tissue scanning first appeared a decade ago, replacing the conventional method of manually cutting ultra-thin slices of tissue one by one to then examine them under a microscope.
The NDP Nanozoomer produces virtual images of full tissue scans which have been analyzed visually as well as by automatic image processing algorithms.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com