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Hodas derided the tissue practice as "aberrant behaviour", saying that engineers usually fear comb filtering and its associated cancellation effects.
All previous studies used protocols noncompliant with current Good Tissue Practice (cGTP) standards, nonclinical-grade reagents, and animal products that made them unfit for clinical use.
Current good tissue practice (cGTP) is intended to prevent human cells, tissues, and cellular and tissue-based products from contamination by infectious disease agents and to ensure that these cells and tissues maintain their integrity and function.
CSC: Cardiac stem cells; FACS: Fluorescence-activated cell sorting; MSCs: Mesenchymal stem cells; aCGH: Array comparative genomic hybridization; RAA: Right atrial appendage; cGMP: Current good manufacturing practice; SOPs: Standard operating procedures; cGTP: Current good tissue practice; bFGF: Basic fibroblast growth factor.
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In January 2001, the agency proposed "good tissue practices," including standards for the manufacture, tracking and labeling of tissue products.
The F.D.A. has proposed new rules for good tissue practices and donor suitability, but it may be several years before they are adopted.
"There are many excellent tissue banks that follow extremely good tissue practices," said Dr. Gary Friedlaender, professor and chairman of the department of orthopedics and rehabilitation at the Yale University School of Medicine, who is a spokesman on tissue bank safety for the American Academy of Orthopedic Surgeons.
The risk of infectious disease transmission emphasizes the need of appropriate sterilization technique in tissue banking practice [ 9].
As imposed by good tissue culture practice, we screened the colon cancer cell lines both for a putative Mollicutes contamination [ 7, 8] and for CD133 expression [ 9, 10].
Contamination with mycoplasma is still prevalent and requires good tissue culture practice and frequent testing to ensure that cell lines are clear of contamination.
Primary cells were isolated from surgically resected DD cord and adjacent, phenotypically normal palmar fascia using routine tissue culture practice as previously described [ 34].
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