Sentence examples for tissue powder from inspiring English sources

Frozen tissue powder (5 g) from 15 shoots were homogenized in 15 ml of 95% ethanol and subsequently centrifuged at 4°C for 10 min at 12000 × g.

For analysis of LOX activity, 4 g of frozen tissue powder from 15 shoots was homogenized in 20 ml of phosphate buffer (pH 7.0).

Frozen tissue powder (4 g) from 15 shoots was homogenized in 20 ml of 0.1 M sodium acetate buffer (pH 5.6).

A total of 285 transient response-time curves containing 34,911 data points were obtained experimentally with four different tissue powder dopamine sensors over a wide range of operating conditions.

The tissue powder and liquid nitrogen were decanted into a cooled tube, and the liquid nitrogen was allowed to evaporate.

The resulting tissue powder was extracted with Trizol (Invitrogen, Carlsbad, CA) reagent according to the manufacturer instructions.

Tissue disruption was performed with a Mikro-Dismembrator in liquid nitrogen and tissue powder (50 to 100 mg) was directly homogeneized in 1 ml of Trizol reagent.

The tissue powder was then resuspended in the cell lysis buffer described above, incubated for 60 min at 4°C and clarified by centrifugation.

Samples were freeze-dried and RNA was extracted from tissue powder using the Pure-Link 96 RNA Kit (Invitrogen) following the manufacturer's instructions.

Tissue powder was lyzed in ice-cold homogenization buffer containing (in mM) Tris (pH: 7.4) 20.0, EDTA 5.0, sucrose 250.0, phenylmethanesulfonyl fluoride 1.0 and 2.5% protease inhibitor cocktail.

Total RNA was isolated from the tissue powder using Trizol Reagent (Invitrogen, CA, USA) and was quantitated using a NanoDrop spectrophometer (Thermo Fisher Scientific Inc., MA, USA).