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Primary cultivation method was improved from the procedures of animal selection, lung tissue perfusion, tissue piece pasting, culture medium selection and so on.
A testicular tissue piece of 7.5mg cryopreserved in cryovial using 1.5M DMSO, an equilibration time of 30 min at 4 °C showed fewer morphological alterations than the other protocols tested.
However, the availability of tissue is unpredictable and every tissue piece is different.
In addition, routine clinical gross and microscopic tissue analysis was complemented with routine research histological examination on the actual tissue piece that was later used for expression analysis.
For the comparison of gene and protein expression in lean versus obese subjects a frozen (−70°C) 300 mg tissue piece was used.
In the end, gross and microscopic clinical evaluation matched the histology of the actual tissue piece being analyzed in 50% of the cancer tissues and 67% of tissues with normal histology.
Similar(33)
The cell-gel constructs were transformed into tissue pieces that were mineralised and contained blood capillaries.
Cells isolated from melanocytic naevus started to growth out of the tissue pieces after a couple of weeks.
In this work, we tested several procedures to process laser microdissected tissue pieces bearing less than 3000 cells.
Immunohistochemistry analysis confirmed the expression of multiple bone markers (osteopontin, osteocalcin, osteonectin and parathyroid hormone receptor) in these tissue pieces.
Following UEA-1 DFL, smooth muscle-myosin and -actin were then visualised by immunolocalisation on the acetone-fixed tissue pieces.
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