Sentence examples for tissue pellets from inspiring English sources

Then, the tissue pellets were homogenized using a PowerGen 125 Tissue Homogenizer (Thermo Fisher Scientific, Waltham, MA) in 100 volumes of PEB (20-600 mM Tris HCl pH 8.0 and 2% SDS).

Tissue pellets were dissolved in 1.0 M NaOH and assayed for protein (Lowry assay).

Tissue pellets were dissolved in 1.0 M NaOH and assayed for protein.

No PrP immunoreactivity was detected in either lichen tissue pellets following homogenization and heating in NuPAGE lithium dodecyl sulfate (LDS) sample buffer with reducing agent.

After the centrifugation at 150g for 15 s, the resulting tissue pellets were dissociated in a 0.6 ml of Neurobasal medium containing 20% FBS by pipeting using the fire polished Pasteur pipet.

For the assessment of the incorporated ECM fraction, tissue pellets were digested with protease K as previously described.

The MeOH supernatant was removed and 200 μL of deionized MilliQ water was then added to the cryo-mill tube containing the homogenized root tissue pellet and vortexed for 30 s.

Tissue pellet were resuspended in RIPA buffer (Pierce) supplemented with protease and phosphatase inhibitor cocktail (Pierce), homogenized for 30 sec on ice and centrifuged at 14,000×g for 15 min at 4°C.

Then, the tissue pellet was air-dried.

The resulting tissue pellet was digested with proteinase K and incubated overnight.

Throughout the procedure, each wash was done by complete resuspending of the tissue pellet.