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The tissue paste was digested in digestion buffer (DMEM/F-12, 1 × gentamicin, 1 × antibiotic-antimycotic (Sigma-Aldrich), 300 U/ml collagenase type III (Worthington Biochemical Corp) and 100 U/ml hyaluronidase (Sigma-Aldrich)) for 1 to 2 hours at 37°C at 125 rpm (10 ml/g tissue).
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Sections of 4 µm were cut from formalin-fixed paraffin-embedded tissues, pasted on glass slides and dried overnight at 37°C.
When I visited the space, it smelled of newly dyed tissue paper and paste and was filled with vibrant crepe paper in reds, yellows, blues, and purples.
Apply the mixture onto the drawing and paste more tissue pieces on it.
Primary cultivation method was improved from the procedures of animal selection, lung tissue perfusion, tissue piece pasting, culture medium selection and so on.
More calcium aluminum hydrate was generated at 3 days, and the pastes structure tissue became dense after 28 days.
Briefly, 8 µm sections of fresh frozen tumor tissue were cryosectioned and pasted on UV sterilized polyethylene naphthalate (PEN) covered glass slides (P.A.L.M. Microlaser Technologies, Bernried, Germany).
Good contact of the electrodes with the tumor tissue was produced using electrocardiography paste (Eko-gel, Camina, Egna, Italy).
The studied nanocrystalline hydroxyapatite paste showed good tissue incorporation.
For added effect, try pasting some colored tissue paper over the wings.
Paste small pieces of tissue paper on the words.
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