Sentence examples for tissue passage from inspiring English sources

Splenocytes were prepared by physical disruption of spleen tissue, passage of the resulting cell suspension through a 40 µm sieve, followed by RBC lysis and resuspension in RPMI 1640 supplemented with 10% FBS and penicillin/streptomycin/fungizone.

After enzymatic digestion of the tissue (passage 0), the cells were grown to 85 to 95% confluence, then trypsinized, counted and 50,000 cells were seeded into five fresh cell culture six-wells (passage 1).

Tumor cells were selected by serial transplant into the cleared mammary fat pads of SCID/Beige immunocompromised mice and the resulting tumor tissue passaged in monolayer and 3D culture for the experiments described herein.

In fact, their growth and revascularization was similar to the growth on the lung tissue prior to being trained via lung tissue passaging.

In addition, this SARS-CoV was of low tissue culture passage, i.e., third passage in Vero E6 cell, which minimized the potential for increased cell culture adaptation and concomitant decrease in vivo replication.

W73 was derived from the 6th tissue culture passage of a strain propagated in fertile hens' eggs, however the passage history prior to this is unknown.

92, state 1); nasal passages are confluent immediately distal to external bony nares (95, 2); right soft tissue nasal passage is oriented dorsoventrally (96, 1); presence of blowhole ligament (101, 1), and presence of premaxillary sacs (105, 1).

This technique involves the use of blunt dilatation to open the pretracheal tissue for passage of the tracheostomy tube.

Using the original or second tissue culture passage would unlikely have resulted in substantial replication in poultry.

This duplication was partially lost in 1 of the 2 isolates after the second BSC40 tissue culture passage.

TEM on the cell pellet of the fourth tissue culture passage from each culture revealed virus particles with an indistinguishable appearance to those in the post-mortem tissues.