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There are spirits present: mask-like black and white vellum paper attached to the top portions of some of the tissue panels.
Figure 6 demonstrates that few, if any, HB1.F3.C1 cells were present in normal brain, kidney, heart, intestine or skin tissue (panels A E, respectively).
In contrast to the modest expression of P-EphA2S897 and LRP1 in GBM tissues, nominal expression was noted in normal brain tissue (panels m and o), and EphA2 expression was similarly weak (panel n).
While quantitative reverse-transcriptase polymerase chain reactions (RT-PCR) can provide more accurate and reproducible results [94], and can be used in conjunction with commercially available human tissue panels, analysis of data from this approach relies on the choice of reference gene for normalization [95].
The tissue panels used in this study did not have enough information about patient survival data.
The multiple tissue panels had been normalised using the mRNA expression levels of several housekeeping genes.
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Furthermore, we used a mouse tissue panel to confirm the antibody specificity.
For the assessment of tissue-specific expression patterns, a commercial tissue panel was employed (Clontech, Palo Alto, CA, USA).
In the analysed tissue panel, only very low levels of hSAA expression were detected in muscle and heart.
No detectable Ep-ICD staining was observed in the normal esophageal tissue (panel C, I), while the ESCC showed intense nuclear and cytoplasmic immunostaining (panel C, II).
We also analyzed the mRNA expression of the different interacting partners of UFM1 in a mouse tissue panel via microarray (Figure 3).
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