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A number of other markers have also been proposed, for example, cotton thread infused with solution of gadopentetate dimeglumine and blue tissue marking dye [9], plastic sheaths of catheter needles [10], cotton thread infused with a solution of Magnevist and blue tissue marking dye [11] and bronze and stainless-steel paints [12].
The area of impact was marked by tissue marking dye.
Tissue marking dye (Polysciences, Warrington, USA) outside the impact area was applied for future reference.
Whole heads were dissected from embryonic mice and individual eyes dissected from P20 mice following labeling of the dorsal edge with Tissue Marking Dye (Cancer Diagnostics) to orientate the eye, fixed in 4% PFA, dehydrated through a graded alcohol series and chloroform and embedded in paraffin.
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The epiblast tissue marks a critical organizational step in the self-assembly of the embryo proper.
The only defect we observed in triple depleted embryos was a marked reduction in blood tissue, marked by Scl.
HoxB9, which is a spinal cord and ventral mesoderm marker (Wright et al, 1990), marks only ventral mesoderm in ventral halves, which are devoid of neural tissue marked by Sox2.
To identify the tissue (marked with "T") in the suture shown in Figure 2H of the ME group, we performed immunohistochemical analysis of Collagen I and found that it was bone tissue, but not residual suture cartilage.
Outgrowing structures observed on 5 g/l yeast food are exclusively composed of PTEN mutant tissue (marked by the absence of GFP) and show high levels of cell death.
Also the TGF-β-superfamily, already detected with the search for GOs sex determination/differentiation, figured among the enriched pathways with "negative regulation of TGF-β receptor signaling pathway" in male tissue (marked green lines in supplementary tables S8 and S9, Supplementary Material online).
Compared to wild-type tissue (marked by GFP in Figure 3c,c'), the number of cleaved-Caspase-3 positive cells is strongly reduced in ark mutant clones suggesting that activation of ΔN-DCP-1 is at least partially dependent on the apoptosome.
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