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Histological results displayed neotissue formations with positive Alcian blue staining in the BMC-articular tissue fragment constructs.
On the basis of his experiments, Child advanced a theory of antero-posterior dominance, stating that physiological activity in a multicellular organism increases along its axis from bottom to top (or tail to head), and that this gradient of activity in a tissue fragment determines the position of structures growing from it.
These minced tissues were washed with PBS and incubated at 37°C in an enzyme solution containing 1.5 mg/ml collagenase + 0.25% trypsin in PBS for 10 30 minutes depending on the size of the tissue fragment.
For some patients the amount of tissue fragment was not enough for all analyses.
For each subcutaneous tumour, one tissue fragment was fixed in formalin before inclusion into paraffin.
The expression of mRNA in a tissue fragment may not necessarily equate with the expression of protein by the tumour.
Similar(29)
Tissue fragments (1 mm3) were fixed with 3% glutaraldehyde plus 0.25% tannic acid in Millonig's buffer containing 0.54%glucose266.
Of the 19,000 bone and tissue fragments taken from the site, Dr. Shaler explains, more than half were so damaged that they did not yield usable DNA results.
The biological constructs containing BMC/BMMSCs and articular tissue fragments were examined in vitro.
Despite worries about decomposition, the Swiss team's report, which was obtained by al-Jazeera and shared with the Guardian prior to publication, found the bone and tissue fragments from the corpse showed "unexpectedly high" levels of radioactive polonium-210.
Scanning electron microscopic images revealed the cells in the constructs with BMC grew and attached into tissue fragments well.
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