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The objective of this in vitro study was to characterize for the first time fibroblast (fibrous scar tissue forming cells) adhesion and proliferation on an important polymeric biomaterial (silicone) coated with titanium using a novel ionic plasma deposition (IPD) process.
In their complexity, the results indicate that Slc39a13 affects the intracellular distribution of Zn in connective tissue forming cells.
We showed SLC39A13 is required for full activation of BMP and TGF-β signaling through controlling the intracellular localization of Smad in connective tissue forming cells.
These results suggested that Slc39a13 was involved in nuclear localization of Smad proteins, but not for their phosphorylation, in connective tissue forming cells.
Our data showed Slc39a13 is involved in BMP/TGF-β signaling pathways in connective tissue forming cells and in nuclear translocation of Smad proteins (Figures 7E, 7F, and S9).
We found that Slc39a13 protein was localized in perinuclear region of osteoblasts, chondrocytes, pulpal cells, and of fibroblasts (Figure 6A), and mainly located in Golgi apparatus (Figure 6B), suggesting that Slc39a13 protein functions as an intracellular Zn transporter in connective tissue forming cells.
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As the PDL precursor cells mature, the role of EGF changes to regulate the differentiation of hard-tissue forming cells and their synthetic activities[19].
Some researchers are already trying to establish new treatments to accelerate the regeneration of periodontal tissue by local application of human recombinant cytokines to stimulate proliferation and differentiation into hard-tissue forming cells of undifferentiated mesenchymal cells among periodontal ligament cells.
As our laboratory focused on bone tissue engineering, regenerating bone tissue with not only bone forming cells but also endothelial cells in order to stimulate angiogenesis rapidly and obtain bone reconstruction simultaneously seems attractive.
Here, we investigate the ontogenetic expression of a suite of genes expressed in shell forming cells and tissues in the tropical abalone Haliotis asinina.
These genes are all expressed in dynamic patterns in shell forming cells and tissues, revealing the complexity of the genetic network underlying molluscan skeletogenesis.
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