Hearts were disrupted by mechanical tissue disruption using a tissue grinder (Polytron).
The amounts of the lachrymatory factor (LF) and pyruvic acid were measured after bulb tissue disruption.
Tissue disruption was enhanced by sonicating three times for 3 s on ice.
In other words, the LF is produced through the successive enzymatic reactions that occur upon onion tissue disruption.
Tissue disruption allows rapid enzymatic degradation of glucosinolates by specific thioglucosidases, denoted myrosinases.
PRENCSO in these lines was not broken down by alliinase and remained after tissue disruption because of the absence of alliinase activity.
Therefore, the decreased levels of pyruvic acid production in #6 and #10 upon tissue disruption may be explained by either a reduced level of PRENCSO or alliinase activity, or both.
Second and third screenings were carried out using M2 and M3 onion bulbs based on the Enzyme-Linked Immunosorbent Assay (ELISA) and the analysis of PRENCSO remaining after tissue disruption as described below.
Plants produce cyanogenic glycosides e.g. prunasin or amygdalin, store these compounds in different tissues, and enzymatically degrade them upon tissue disruption, in order to finally release toxic hydrogen cyanide2.
This work seeks to establish the feasibility of characterizing the ultrastructure of brain tissue disruption associated with the implantation of carbon fiber voltammetric microelectrodes.
Thermal treatment (boiling) significantly increased carotenoid bioaccessibility from the tomatoes digested with emulsion, which was attributed to plant tissue disruption at elevated temperatures facilitating carotenoid release (p < 0.05).
