Sentence examples for tissue dishes from inspiring English sources

Cells were plated on 24-well tissue culture plates at 1×106 cells/well for supernatant analysis or on 100 cm tissue dishes at 2.5×107 cells/dish for protein analysis.

Briefly, a total of 2×106 bone marrow cells were cultured in tissue dishes containing 10 ml of RPMI 1640 supplemented with 2 mM glutamine, 1% nonessential amino acids (Gibco-BRL), 10% FCS, 50 ng/ml of GM-CSF and 50 ng/ml IL-4 (PeproTech Inc).

The resuspended human mesothelial cells (HMCs) were cultivated in Medium 199 (PAA Laboratory GmbH, Pasching, Austria) in fibronectin- (Sigma-Aldrich, Taufkirchen, Germany) coated tissue dishes.

CRL-1825) were cultured on gelatinized tissue dishes in Dulbecco's modified Eagle's medium (DMEM) containing 10% foetal calf serum, 0.05 m mβ-mercaptoethanol, 100 IU/mL penicillin, and 0.1 mg/mL streptomycin.

This cell line was cultured in Keratinocyte-SFM supplemented with 50 μg/mL bovine pituitary extract and 5 ng/mL epidermal growth factor (Gibco) at 37 °C on porcine gelatin-coated tissue dishes as previously described [ 22].

For siRNA transfection, Me30966 and WM793 cells were seeded in unbuffered medium at 30% confluence in 12-well tissue dishes transfected with 5 nM of specific siRNA by using Interferin (PolyPlusTransfection, Lafayette, CO, USA) following the manufacturer's protocol.

Cells were plated in 10 cm tissue culture dishes (1 cultured dish per embryo) in standard media.

Cells were immediately seeded into 35 mm tissue culture dishes at 1.0x10 cells per dish.

Cells were seeded in 100 mm tissue culture dishes (5 × 10 cells per dish) and cultured to ∼80% confluency.

C3H10T1/2 cells of 1.0 × 10 were cultured on PAMPS/PDMAAm DN gel in polystyrene tissue culture dishes or directly on polystyrene tissue culture dishes.

RNAi treatments were performed in six-well tissue culture dishes (Olympus Plastics) or 10-cm tissue culture dishes (Corning) by addition of 10 µg/mL double-stranded RNA (dsRNA) to cells plated at 70 90% confluency.