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Only 55 of the detected metabolites overlapped with LycoCyc, indicating incompleteness of the LycoCyc database, difficulties arising from the high number of similar but not identical metabolites in plant metabolism, and the tissue dependency of metabolite occurrences.
It has also been shown that alternative splicing in cancer exhibits both tissue dependency and dependency upon the type of splicing event considered [ 21].
This sort of tissue dependency not only allows the identification of genes active in the respective tissues with different mechanisms of transcriptional control (eQTLs), but also provides insight in organ dependent regulation which is the basis for tissue specific network reconstructions.
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Via multidimensional unfolding analysis, we visualized this tissue-dependency.
Apart from limitations that are generally associated with cadaveric measurements, such as post-mortem degeneration of tissue, age-dependency and effects of cadaver freezing, this study is based on measurements of only four cadaveric knee joints.
Summary of sample sizes per chip platform and brain tissue and data dependency structure with respect to donor subjects are presented.
When DO2 falls below a critical low value, a proportionate increase in OE cannot be maintained and the VO2 starts to fall (oxygen supply dependency) and tissue hypoxia occurs as reflected by an abrupt increase in blood lactate concentration [13 15].
One difficulty comes from tissue and cell type dependency of gene expression.
To explain its heterogeneous behavior, PTEN has been recently appointed as a haploinsufficient gene, characterized, however, by tissue specificity and context dependency [ 19].
When investigating the dependency between tissue composition and the number of unassigned samples, we observed a higher number of unassigned cases where samples contained similar proportions of cancer and normal tissue (proportions of 50/50, 60/40 and 40/60).
Of note, in contrast with plasma and brain tissue, in which dose dependency was somewhat linear, increasing quinacrine doses in spleen and liver were associated with substantially greater tissue levels.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com